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Whitepapers

Production of Stem Cells in Single-use Vessels

September 30, 2014

In this study, the success of large clinical-scale culture of human adipose-derived mesenchymal stem cells (AdMSCs) in an industrial single-use vessel at 3.75 L working volume is demonstrated. The vessel offers a precision-controlled environment for the ideal growth of stem cells under simulated physiological conditions.

High-Resolution Separation of a Fusion Protein

September 30, 2014

A high-resolution HIC application for the separation of fusion protein therapeutics. This application, based on protein surface hydrophobicity, allows separation of a fusion protein from a truncated version and structural isomer.

Mammalian Virus Purification Using Ceramic Hydroxyapatite

September 30, 2014

Conventional techniques for mammalian virus purification produce variable quality, quantity, and significant loss of particle infectivity. We propose the chromatographic separation of viral particles of diverse sizes and from different families such as dengue, influenza, mouse hepatitis, adenovirus, poliovirus using CHT Ceramic Hydroxyapatite resin.

PEGylated Protein Analysis by Size-Exclusion and Reversed-Phase UPLC

September 30, 2014

The first PEGylated biotherapeutic, pegademase, which is a bioconjugate of the bovine derived enzyme adenosine deaminase and 5 KDa molecular weight (MW) polyethylene glycol (PEG), was introduced in 1990. Pegademase is used for the treatment of individuals with severe combined immunodeficiency disease (SCID).

Efficient, high-titer monoclonal antibody production in a fed-batch process using single-use stirred-tank and rocking bioreactor systems

September 30, 2014

MAb production in fed-batch processes with single-use Xcellerex™ XDR-200 stirred-tank and ReadyToProcess WAVE™ 25 rocking bioreactor systems. Cell expansion was performed in a 15 L rocking bioreactor culture to seed a 200 L stirred-tank bioreactor culture.

More Efficiency with Equivalent Data: MFI 5200

September 30, 2014

Micro-Flow Imaging (MFI) is now the go-to technology for monitoring and characterizing a wide range of visible and subvisible particles and protein aggregates using particle size and morphology. This application note shows how the latest MFI system stacks up to its predecessor by cutting the hands-on time for sample analysis without any loss in functionality and performance.

Minimizing Excipient Fluorescence Interference Using Handheld Raman

September 30, 2014

A study of commonly used pharmaceutical excipients concluded that 1064nm handheld Raman overcomes sample generated fluorescence, thus providing compound specific “signature” Raman peaks for more accurate identification.

High-Resolution Separation of Cysteine-Conjugated Antibody Drug Mimics

August 26, 2014

Cysteine-conjugated ADC mimics are successfully separated based on their drug-to-antibody ratio (DAR) using a Thermo Scientific™ MAbPac™ HIC-10 column. Separation of ADC mimics was optimized by adjusting temperature, solvent content, and gradient.

Enhanced Assurance of Supply for Single-Use Bags

August 26, 2014

Enhanced assurance of supply for single-use bags is based on material science, quality by design, and partnership with suppliers.

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