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Whitepapers

Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures

March 25, 2014

To monitor monoclonal antibody titer and yield from cell-culture supernatants before expensive prep and large amounts of Protein A are employed, an analytical scale procedure helps determine the titer of monoclonal antibody for the optimal time for harvest of the mAb product. Here, pre-packed Agilent Bio-Monolith Protein A columns quickly capture mAb titer from cell supernatant.

Peptide Mapping of Glycoprotein Erythropoietin by HILIC LC/MS and RP-LC/MS

March 25, 2014

In this application note an Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300-HILIC 1.8 ?m column and an Agilent AdvanceBio Peptide Mapping column are used in combination with time of flight (TOF) mass-spectrometry (MS) for mapping EPO protein. The work demonstrates the utility of HILIC as an orthogonal and complementary approach to reversed-phase LC/MS for peptide analysis.

Fast and Efficient Peptide Mapping of a Monoclonal Antibody (mAb): UHPLC Performance Using Superficially Porous Particles

March 25, 2014

In this work, an AdvanceBio Peptide Mapping column is used to generate a rapid and highly efficient peptide map at a traditional LC system pressure. The column achieves substantial improvements in peptide mapping during very fast run times and low system pressures, while still maintaining high peak-performance efficiency.

Confirming Accurate Particle Counting and Sizing on MFI Systems

February 25, 2014

Micro-Flow Imaging (MFI) provides accurate and sensitive detection of sub-visible particles and protein aggregates in biopharmaceuticals. Confirmation of instrument performance is demonstrated using NIST-traceable polystyrene beads. This application note provides a guide to best practices for handling standards and samples to ensure sensitive, accurate, and repeatable results.

Charge Variant Analysis using pH Gradients

January 28, 2014

This Application Note shows method development for charge variant profiling of monoclonal antibodies using pH gradient elution with Agilent Buffer Advisor Software and online pH and conductivity monitoring.

LC/MS Analysis of the Monoclonal Antibody Rituximab

January 28, 2014

In this Application Note, an Orbitrap-based mass spectrometer is used for high-confidence analysis of the intact and reduced forms of rituximab (monoclonal antibody), in biopharmaceutical product development and characterization.

How to Conduct Better Stability Studies with FDA/ICH Guidance

January 28, 2014

Download the new Application Note: “Better Stability Studies: Match Your Environmental Monitoring & Mapping to FDA/ICH Guidance.” Learn how to minimize the risk of incomplete data from result power or network failures during environmental mapping or monitoring applications and ensure stability study data complies with FDA/ICH guidance.

Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein Separations

January 28, 2014

For HIC separations, parameters other than resin surface modifications can be employed to enhance performance. This application note addresses the electrolyte composition of the mobile phase as one parameter responsible for protein adsorption and desorption. The results presented illustrate the benefits regarding capacity and selectivity in HIC of often neglected salts and their mixtures.

Tosoh - Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein Separations

January 27, 2014

Tosoh - Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein Separations

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