Whitepapers - BioPharm International

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Whitepapers

TOYOPEARL GigaCap DEAE-650M Performance Comparison to TOYOPEARL DEAE-650M

July 30, 2013

TOYOPEARL GigaCap DEAE-650M is a very high capacity anion exchange resin capable of delivering products of similar purity to that seen with the TOYOPEARL DEAE-650M resin with identical operating conditions yet is more tolerant of higher conductivities.

Analysis of Polyethylene Glycol (PEG) and a Mono and Di-PEGylated Therapeutic Protein Using HPLC and Q-TOF Mass Spectrometry

May 28, 2013

A method using LC/MS is described for improving the analysis PEGylated peptides and proteins using the post-column addition of a charge stripping agent to obtain simpler mass spectra for detailed structural characterization.

Automated Dynamic Light Scattering for Aggregation Detection

May 28, 2013

Promiscuous non-stoichiometric inhibition can be a serious problem when screening libraries of compounds in a high-throughput format. A rapid method of detecting such compound behavior can be achieved using a DynaPro Plate Reader to examine the light scattering properties of a dilution series of known aggregating inhibitors.

Automated Fiber Particle Imaging and Characterization Including Differentiating Fiber Curl from Straightness

May 28, 2013

This application note details how imaging particle analysis automatically characterizes fibrous particles using the actual image and shape in addition to ESD. This allows each, individual fiber to be accurately measured for the degree of curl, straightness, length, width and other parameters, and to be automatically differentiated from each other.

Driving Innovation in BioPharma with Product Lifecycle Management

May 28, 2013

This white paper explores how product lifecycle management (PLM) can enable biopharma companies to develop innovative products that generate real economic benefits for their organizations and health benefits for patients.

Reconstitution of a High-Concentration Protein Drug Product

May 28, 2013

A case study reviews the challenges in reducing reconstitution time of a recombinant protein. The example may provide useful information and practical guidelines for the development of other high-concentration protein drug products.

Purification of Oligonucleotides on TOYOPEARL GigaCap® Q-650S

May 28, 2013

TOYOPEARL GigaCap Q-650S is capable of delivering oligonucleotides of comparable purity to that seen with the TSKgel® SuperQ-5PW (20) resin and at slightly higher process yields under the same loading conditions but at lower pressures.

Characterization of Glycosylation in the Fc Region of Therapeutic Recombinant Monoclonal Antibody

May 28, 2013

In this note, we describe a fast and efficient liquid chromatography-TOF mass spectrometry approach to characterize an IgG derived from CHO cells. An Agilent Poroshell 300SB-C3, 5 um superficially porous IgG1 column was used to obtain accurate glycoform masses of an intact IgG1, with a subsequent analysis of papain-digeste3d IgG1 to obtain site-specific glycosylation profile information of the Fc region for further characterization of IgG1 heterogeneity. Additionally, a subsequent mAb-glyco chip LC/MS analysis was used to build a glycan accurate mass database for additional validation of the glycans identified in the TOF mass analysis.

Protein Separation with pH Gradients Using Composite Buffer Systems

April 30, 2013

Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.

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