Whitepapers - BioPharm International



Liposome Characterization by FFF-MALS-QELS

April 30, 2013

Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing and quality control, it is of great importance to monitor liposome size and encapsulation. Field flow fractionation (FFF) with the concomitant use of Multi-Angle Light Scattering (MALS) and Quasi-Elastic Light Scattering (QELS, aka dynamic light scattering) is an ideal tool for such characterization.

Purification of Recombinant Proteins: A Simple Approach to Method Development

February 25, 2014

Using a simple DOE exercise, we demonstrate how to elucidate the nature and degree of interaction between various proteins and the binding ligand for Nuvia™ cPrime™ Hydrophobic Cation Exchange Media. Thus, allowing method developers to quickly and effectively optimize selectivity and define design space parameters

Ligand Binding Analysis Using Surface Plasmon Resonance

February 25, 2014

When ligand binding is part of the functional activity of a biopharmaceutical, FDA and EMA guidelines require this property be quantified. The Biacore T200 provides real-time and label-free biomolecular interaction analysis and binding assays.

Are “Canned” Performance Qualification Protocols Ok?

February 25, 2014

What’s the quickest solution to a robust PQ of your environmental monitoring system: DIY or vendor supplied? This article discusses the keys to evaluating “canned” PQ documents from suppliers and options for creating your own protocols. Also, is skipping the PQ process an option? Learn more…

Separating Beyond Question

December 27, 2013

ACQUITY QDa Detector is as intuitive as an optical detector, with the robustness to handle all of your analyses. Working in harmony with your chromatography, it is pre-optimized to work with your samples, without the sample-specific or user adjustments typical of traditional mass spectrometers.

Affinity Removal High Abundant Proteins in Human Plasma

December 27, 2013

This Application Note describes the depletion of high abundant proteins from plasma using the Agilent Multiple Affinity Removal System coupled with the Agilent 1260 Infinity Bio-inert Quaternary LC.

Separation of Impurities in a Single Step using a Mixed-Mode Chromatography Media

December 27, 2013

MPC™ media is a versatile mixed-mode chromatography media with unique separation properties for multiple polishing applications at process scale. Combined properties of hydroxyapatite and fluoroapatite provides superior clearance performance.

Monoclonal Antibody Purification with a High Capacity Protein A Resin

November 26, 2013

Protein A resins constitute a substantial cost in state-of-the-art mAb purification processes. Factors such as operating cycles, capacity, and mAb titer can have an impact on total costs associated with mAb purification. The purification of a monoclonal antibody from crude feed stock using TOYOPEARL? AF-rProtein A HC-650F, a high capacity protein A resin from Tosoh Bioscience, show that this particular high capacity protein A resin delivers highly pure antibodies at yields approaching 90%.

Detailed Aggregation Characterization of Monoclonal Antibodies

April 29, 2014

This Application Note shows detailed size exclusion analysis of monoclonal antibodies and their aggregates using the Agilent 1260 Infinity Multi-Detector Bio-SEC Solution.



Click here