Whitepapers - BioPharm International

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Whitepapers

Thresholding for Accurate Detection and Measurement of Transparent Particles such as Protein Agglomerates

April 30, 2013

This paper explains how adjusting the gray-scale threshold value using both darker and lighter pixels reveals transparent particles that would otherwise escape detection to ensure accurate count and concentration calculations. Protein agglomerate images demonstrate how properly varying threshold documents presence of one, large particle instead of several small particles.

Liposome Characterization by FFF-MALS-QELS

April 30, 2013

Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing and quality control, it is of great importance to monitor liposome size and encapsulation. Field flow fractionation (FFF) with the concomitant use of Multi-Angle Light Scattering (MALS) and Quasi-Elastic Light Scattering (QELS, aka dynamic light scattering) is an ideal tool for such characterization.

TOYOPEARL GigaCap® DEAE-650M Preserving Resolution at Increased Protein Loads

April 30, 2013

TOYOPEARL GigaCap DEAE-650M, a very high capacity anion exchange resin for process scale chromatography, offers chromatographers a combination of binding capacities greater than 150 g/L while maintaining peak resolution.

Dual Wavelength 785 nm and 1064 nm Excitation Portable Raman Spectrometer: Rapid Authentication of Genuine and Counterfeit Pharmaceuticals

April 30, 2013

Overcoming fluorescence interference in counterfeit pharmaceutical samples is achieved using a dispersive, dual wavelength Xantus®-2 portable Raman spectrometer, equipped with both 785 nm and 1064 nm laser excitation. The dual wavelength system affords the opportunity for selection of an appropriate excitation for rapid identification of a wide range of genuine and counterfeit pharmaceuticals.

Separation of Impurities in a Single Step using a Mixed-Mode Chromatography Media

December 27, 2013

MPC™ media is a versatile mixed-mode chromatography media with unique separation properties for multiple polishing applications at process scale. Combined properties of hydroxyapatite and fluoroapatite provides superior clearance performance.

Separating Beyond Question

December 27, 2013

ACQUITY QDa Detector is as intuitive as an optical detector, with the robustness to handle all of your analyses. Working in harmony with your chromatography, it is pre-optimized to work with your samples, without the sample-specific or user adjustments typical of traditional mass spectrometers.

Affinity Removal High Abundant Proteins in Human Plasma

December 27, 2013

This Application Note describes the depletion of high abundant proteins from plasma using the Agilent Multiple Affinity Removal System coupled with the Agilent 1260 Infinity Bio-inert Quaternary LC.

Monoclonal Antibody Purification with a High Capacity Protein A Resin

November 26, 2013

Protein A resins constitute a substantial cost in state-of-the-art mAb purification processes. Factors such as operating cycles, capacity, and mAb titer can have an impact on total costs associated with mAb purification. The purification of a monoclonal antibody from crude feed stock using TOYOPEARL? AF-rProtein A HC-650F, a high capacity protein A resin from Tosoh Bioscience, show that this particular high capacity protein A resin delivers highly pure antibodies at yields approaching 90%.

10-Minute Charge Heterogeneity Analysis

December 27, 2013

Platform methods, high resolution, and ease of use have made the iCE system the gold standard for protein charge heterogeneity characterization for biopharmaceuticals. At 15-18 minutes per sample, iCE methods are already fast and simple but now they are even better. In this application note we describe a high throughput 10-minute charge heterogeneity analysis method and performance improvements that provide automated analysis of 100 samples at a time.

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