Whitepapers - BioPharm International

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Whitepapers

Tosoh - Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein Separations

January 27, 2014

Tosoh - Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein Separations

10 Minute Charge Heterogeneity Analysis

January 2, 2014

Platform methods, high resolution and ease of use have made the iCE system the gold standard for protein charge heterogeneity characterization for biopharmaceuticals. At 15-18 minutes per sample, iCE methods are already fast and simple but now they are even better. In this application note we describe a high throughput 10 minute charge heterogeneity analysis method and performance improvements that provide automated analysis of 100 samples at a time.

Protein Separation with pH Gradients Using Composite Buffer Systems

April 30, 2013

Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.

Liposome Characterization by FFF-MALS-QELS

April 30, 2013

Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing and quality control, it is of great importance to monitor liposome size and encapsulation. Field flow fractionation (FFF) with the concomitant use of Multi-Angle Light Scattering (MALS) and Quasi-Elastic Light Scattering (QELS, aka dynamic light scattering) is an ideal tool for such characterization.

Dual Wavelength 785 nm and 1064 nm Excitation Portable Raman Spectrometer: Rapid Authentication of Genuine and Counterfeit Pharmaceuticals

April 30, 2013

Overcoming fluorescence interference in counterfeit pharmaceutical samples is achieved using a dispersive, dual wavelength Xantus®-2 portable Raman spectrometer, equipped with both 785 nm and 1064 nm laser excitation. The dual wavelength system affords the opportunity for selection of an appropriate excitation for rapid identification of a wide range of genuine and counterfeit pharmaceuticals.

TOYOPEARL GigaCap® DEAE-650M Preserving Resolution at Increased Protein Loads

April 30, 2013

TOYOPEARL GigaCap DEAE-650M, a very high capacity anion exchange resin for process scale chromatography, offers chromatographers a combination of binding capacities greater than 150 g/L while maintaining peak resolution.

Thresholding for Accurate Detection and Measurement of Transparent Particles such as Protein Agglomerates

April 30, 2013

This paper explains how adjusting the gray-scale threshold value using both darker and lighter pixels reveals transparent particles that would otherwise escape detection to ensure accurate count and concentration calculations. Protein agglomerate images demonstrate how properly varying threshold documents presence of one, large particle instead of several small particles.

Purification of Recombinant Proteins: A Simple Approach to Method Development

February 25, 2014

Using a simple DOE exercise, we demonstrate how to elucidate the nature and degree of interaction between various proteins and the binding ligand for Nuvia™ cPrime™ Hydrophobic Cation Exchange Media. Thus, allowing method developers to quickly and effectively optimize selectivity and define design space parameters

Ligand Binding Analysis Using Surface Plasmon Resonance

February 25, 2014

When ligand binding is part of the functional activity of a biopharmaceutical, FDA and EMA guidelines require this property be quantified. The Biacore T200 provides real-time and label-free biomolecular interaction analysis and binding assays.

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