Whitepapers - BioPharm International

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Whitepapers

Dual Wavelength 785 nm and 1064 nm Excitation Portable Raman Spectrometer: Rapid Authentication of Genuine and Counterfeit Pharmaceuticals

April 30, 2013

Overcoming fluorescence interference in counterfeit pharmaceutical samples is achieved using a dispersive, dual wavelength Xantus®-2 portable Raman spectrometer, equipped with both 785 nm and 1064 nm laser excitation. The dual wavelength system affords the opportunity for selection of an appropriate excitation for rapid identification of a wide range of genuine and counterfeit pharmaceuticals.

Thresholding for Accurate Detection and Measurement of Transparent Particles such as Protein Agglomerates

April 30, 2013

This paper explains how adjusting the gray-scale threshold value using both darker and lighter pixels reveals transparent particles that would otherwise escape detection to ensure accurate count and concentration calculations. Protein agglomerate images demonstrate how properly varying threshold documents presence of one, large particle instead of several small particles.

Protein Separation with pH Gradients Using Composite Buffer Systems

April 30, 2013

Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.

Liposome Characterization by FFF-MALS-QELS

April 30, 2013

Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing and quality control, it is of great importance to monitor liposome size and encapsulation. Field flow fractionation (FFF) with the concomitant use of Multi-Angle Light Scattering (MALS) and Quasi-Elastic Light Scattering (QELS, aka dynamic light scattering) is an ideal tool for such characterization.

Ligand Binding Analysis Using Surface Plasmon Resonance

February 25, 2014

When ligand binding is part of the functional activity of a biopharmaceutical, FDA and EMA guidelines require this property be quantified. The Biacore T200 provides real-time and label-free biomolecular interaction analysis and binding assays.

Are “Canned” Performance Qualification Protocols Ok?

February 25, 2014

What’s the quickest solution to a robust PQ of your environmental monitoring system: DIY or vendor supplied? This article discusses the keys to evaluating “canned” PQ documents from suppliers and options for creating your own protocols. Also, is skipping the PQ process an option? Learn more…

Purification of Recombinant Proteins: A Simple Approach to Method Development

February 25, 2014

Using a simple DOE exercise, we demonstrate how to elucidate the nature and degree of interaction between various proteins and the binding ligand for Nuvia™ cPrime™ Hydrophobic Cation Exchange Media. Thus, allowing method developers to quickly and effectively optimize selectivity and define design space parameters

Affinity Removal High Abundant Proteins in Human Plasma

December 27, 2013

This Application Note describes the depletion of high abundant proteins from plasma using the Agilent Multiple Affinity Removal System coupled with the Agilent 1260 Infinity Bio-inert Quaternary LC.

Separating Beyond Question

December 27, 2013

ACQUITY QDa Detector is as intuitive as an optical detector, with the robustness to handle all of your analyses. Working in harmony with your chromatography, it is pre-optimized to work with your samples, without the sample-specific or user adjustments typical of traditional mass spectrometers.

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