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Whitepapers

Analysis of Erythropoietin Glycopeptides and Glycoforms by HILIC and RP Chromatography

May 28, 2013

Reversed-phase UHPLC/HPLC is routinely used for peptide mapping, but if the digest contains hydrophilic peptides, such as glycopeptides, valuable information can be missed. This note demonstrates peptide mapping of digested glycoprotein erythropoietin (EPO) protein using an Agilent ZORBAX Rapid Resolution High Definition 300-HILIC 1.8 ?m LC column and a TOF MS. Taking advantage of the high organic solvent system of the mobile phase for HILIC (hydrophilic interaction chromatography), the glycopeptides are effectively dissolved, retained and separated by the HILIC column. This app note includes the separation, sequence coverage and comparison of reversed-phase and HILIC data to demonstrate the utility of HILIC as an orthogonal and complementary approach to RPLC/MS for peptide analysis.

HarvestClearTM Filtration System for harvest of small-scale bioreactors

May 1, 2013

The clarification of batch and fed-batch bioreactor systems can pose a practical challenge to biopharmaceutical manufacturers due to high cell densities. The HarvestClearTM Filtration System is an automated single-use system designed to optimize clarification of 1L to 20L high density bioreactor outputs, while reducing hands-on time and increasing operator safety.

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