MPC™ media is a versatile mixed-mode chromatography media with unique separation properties for multiple polishing applications at process scale. Combined properties of hydroxyapatite and fluoroapatite provides superior clearance performance.
Nuvia™ HR-S media is a new cation exchanger designed for high resolution of closely related product impurities such as aggregates. It delivers excellent resolution with a final aggregate content of < 0.3% and a high recovery of > 80% from a heterogeneous feed of monoclonal antibody aggregates and monomer. Aggregate content and recovery in the eluate were shown to be a function of the target conductivity measured at the end of collection
Using a simple DOE exercise, the authors demonstrate how to elucidate the nature and degree of interaction between various proteins and the binding ligand for Nuvia™ cPrime™ Hydrophobic Cation Exchange Media, thus allowing method developers to quickly and effectively optimize selectivity and define design space parameters.
Monoclonal antibodies are currently the most important class of therapeutic proteins. This application demonstrates that a three-step nonaffinity workflow can effectively deliver highly purified monoclonal antibodies with minimal feed conditioning.
Process Chromatography columns must provide a very high level of cleanability to meet the requirements of various regulatory agencies. This study details the sanitary design and cleaning procedure of the Bio-Rad InPlace column.