Articles by Thermo Fisher Scientific - BioPharm International

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Articles by Thermo Fisher Scientific

Thermo Fisher Scientific


Articles
Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD
May 26, 2014

By combining HPAE-PAD analysis with acid hydrolysis and enzymatic digestion of protein glycans, information about the glycan identity as well as terminal carbohydrate linkage isomers can be determined from small amounts of protein.

LC/MS Analysis of the Monoclonal Antibody Rituximab
January 28, 2014

In this Application Note, an Orbitrap-based mass spectrometer is used for high-confidence analysis of the intact and reduced forms of rituximab (monoclonal antibody), in biopharmaceutical product development and characterization.

A Novel Affinity Protein A Column for Monoclonal Antibody (MAb) Titer Analysis
October 29, 2013

The Thermo Scientific™ MAbPac™ Protein A column is a high-performance affinity chromatography column specifically designed for the determination of monoclonal antibody concentration in harvest cell culture. The nonporous, hydrophilic stationary phase enables fast and accurate MAb titer analysis. The ability to collect the IgG fraction during the HPLC analysis allows the analyst to characterize the IgG charge variants using a novel pH gradient approach.

Novel pH Gradient Separation Platform for Monoclonal Antibody Charge Variant Analysis
July 30, 2013

A novel pH gradient method for cation-exchange chromatography is discussed. Linear pH gradient was achieved using a multi-component buffer system containing multiple zwitterionic buffer species with pI values from 6 to 10.

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