Articles by Thermo Fisher Scientific - BioPharm International



Articles by Thermo Fisher Scientific

Thermo Fisher Scientific

Carbohydrates Analysis Application Notebook
November 25, 2014

The Carbohydrate Analysis Application Notebook consists of applications using HPAE-PAD for the analysis of glycans, carbohydrates in food and beverages, and carbohydrates in biofuels.

High-Throughput, High-Resolution mAb Analysis with Small Particle Size HPLC Columns
October 28, 2014

High-throughput applications of the Thermo Scientific™ MAbPac™ SCX-10 column, showing acidic and basic variant analysis, including mAb lysine truncation variant characterization. Ruggedness of the 3 μm small particle phase column is also demonstrated.

High-Resolution Separation of a Fusion Protein
September 30, 2014

A high-resolution HIC application for the separation of fusion protein therapeutics. This application, based on protein surface hydrophobicity, allows separation of a fusion protein from a truncated version and structural isomer.

High-Resolution Separation of Cysteine-Conjugated Antibody Drug Mimics
August 26, 2014

Cysteine-conjugated ADC mimics are successfully separated based on their drug-to-antibody ratio (DAR) using a Thermo Scientific™ MAbPac™ HIC-10 column. Separation of ADC mimics was optimized by adjusting temperature, solvent content, and gradient.

Rapid Separation and Mass Spectrometry Detection of Intact Protein Samples Using Monolithic Capillary Columns
July 29, 2014

This application note demonstrates the rapid separation of a wide mass range of peptides and proteins using a reversed-phase monolithic column with both UV and mass spectrometry (MS) detection. The separation is conducted on Thermo Scientific™ ProSwift™ RP-4H columns using conventional water/acetonitrile-based eluents. These simple, straightforward methods can be used to optimize chromatographic separations for analysis time, resolution, and MS detection especially for top-down proteomics.

Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD
May 26, 2014

By combining HPAE-PAD analysis with acid hydrolysis and enzymatic digestion of protein glycans, information about the glycan identity as well as terminal carbohydrate linkage isomers can be determined from small amounts of protein.

LC/MS Analysis of the Monoclonal Antibody Rituximab
January 28, 2014

In this Application Note, an Orbitrap-based mass spectrometer is used for high-confidence analysis of the intact and reduced forms of rituximab (monoclonal antibody), in biopharmaceutical product development and characterization.



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