Articles by Thermo Fisher Scientific - BioPharm International

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Articles by Thermo Fisher Scientific

Thermo Fisher Scientific


Articles
High-Resolution Separation of a Fusion Protein
September 30, 2014

A high-resolution HIC application for the separation of fusion protein therapeutics. This application, based on protein surface hydrophobicity, allows separation of a fusion protein from a truncated version and structural isomer.

High-Resolution Separation of Cysteine-Conjugated Antibody Drug Mimics
August 26, 2014

Cysteine-conjugated ADC mimics are successfully separated based on their drug-to-antibody ratio (DAR) using a Thermo Scientific™ MAbPac™ HIC-10 column. Separation of ADC mimics was optimized by adjusting temperature, solvent content, and gradient.

Rapid Separation and Mass Spectrometry Detection of Intact Protein Samples Using Monolithic Capillary Columns
July 29, 2014

This application note demonstrates the rapid separation of a wide mass range of peptides and proteins using a reversed-phase monolithic column with both UV and mass spectrometry (MS) detection. The separation is conducted on Thermo Scientific™ ProSwift™ RP-4H columns using conventional water/acetonitrile-based eluents. These simple, straightforward methods can be used to optimize chromatographic separations for analysis time, resolution, and MS detection especially for top-down proteomics.

Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD
May 26, 2014

By combining HPAE-PAD analysis with acid hydrolysis and enzymatic digestion of protein glycans, information about the glycan identity as well as terminal carbohydrate linkage isomers can be determined from small amounts of protein.

LC/MS Analysis of the Monoclonal Antibody Rituximab
January 28, 2014

In this Application Note, an Orbitrap-based mass spectrometer is used for high-confidence analysis of the intact and reduced forms of rituximab (monoclonal antibody), in biopharmaceutical product development and characterization.

A Novel Affinity Protein A Column for Monoclonal Antibody (MAb) Titer Analysis
October 29, 2013

The Thermo Scientific™ MAbPac™ Protein A column is a high-performance affinity chromatography column specifically designed for the determination of monoclonal antibody concentration in harvest cell culture. The nonporous, hydrophilic stationary phase enables fast and accurate MAb titer analysis. The ability to collect the IgG fraction during the HPLC analysis allows the analyst to characterize the IgG charge variants using a novel pH gradient approach.

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