Articles by Phu T. Duong - BioPharm International

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Articles by Phu T. Duong

Phu T. Duong


Articles
Peptide Mapping of Glycoprotein Erythropoietin by HILIC LC/MS and RP-LC/MS
March 25, 2014

In this application note an Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300-HILIC 1.8 ?m column and an Agilent AdvanceBio Peptide Mapping column are used in combination with time of flight (TOF) mass-spectrometry (MS) for mapping EPO protein. The work demonstrates the utility of HILIC as an orthogonal and complementary approach to reversed-phase LC/MS for peptide analysis.

Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures
March 25, 2014

To monitor monoclonal antibody titer and yield from cell-culture supernatants before expensive prep and large amounts of Protein A are employed, an analytical scale procedure helps determine the titer of monoclonal antibody for the optimal time for harvest of the mAb product. Here, pre-packed Agilent Bio-Monolith Protein A columns quickly capture mAb titer from cell supernatant.

Does Tween 20 Affect Monoclonal Antibody Separation?
October 29, 2013

Tween 20 has various beneficial properties for biological analysis, such as stabilizing proteins, solubilizing protein membranes, and preventing nonspecific binding of primary and secondary antibodies. Therefore, Tween 20 is often present in samples of monoclonal antibodies that require analysis using techniques such as SEC. This app note investigates whether the presence of Tween 20 detergent directly in the sample and in the eluent during multiple injections of an IgG sample onto an Agilent Bio SEC column affects the analysis or the columns.

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