Innovations and Future Trends in HPLC Column Technology - Pharmaceutical Technology

Latest Issue

Latest Issue
PharmTech Europe

Innovations and Future Trends in HPLC Column Technology

Pharmaceutical Technology Europe
Volume 26, Issue 8, pp. 45
PharmTech Europe 25th Anniversary
DSGpro/Getty Images;
Art direction: Dan Ward

From QC to QbD and Beyond

Analytical technologies and bio/pharmaceutical process development have advanced in parallel through necessity and innovation.

An average high performance liquid chromatography (HPLC) instrument uses 6-8 columns per year; as the number of instruments grows, so does the market for columns. The overall market for columns (analytical, preparative/process, capillary/nano, bulk packing, and accessories) is now estimated to be $1.3 billion with an overall growth of 3.5% (1). Growth in the high performance liquid chromatography (UHPLC) segment is higher. Developments in column technology to deliver greater efficiency, speed and inertness benefit the drug development process from discovery to manufacturing and quality control.

Pharmaceutical Technology Europe
25th Anniversary Issue

Innovations and Future Trends in HPLC Column Technology (Expanded Coverage)

Advances in the Pharmaceutical Industry Have Shaped Particle Sizing Technology

For new methods, superficially porous particle (SPP) columns have become the favoured column type in pharmaceutical laboratories thanks to the lower pressure, enhanced efficiency and equal loadability offered in comparison to smaller totally porous particles. The development of even smaller SPP particles can also be anticipated. If SPP columns continue to dominate, the need to further increase instrument pressure limits may not be necessary, but in chromatography, pressure is always a useful commodity.

If researchers are able to improve efficiency without great increases in backpressure and can make longer length columns for difficult separations, monolithic columns will still have great promise. In particular, polymeric monoliths could be quite attractive because their wider operating range gives them some advantages. Monoliths may become the favoured approach for laboratory-on-a-chip systems since they can be synthesized in situ inside the narrow channels where efficient packing of particulates may prove exceedingly difficult.

With its orthogonal separation power, supercritical-fluid chromatography (SFC) has made a comeback in the rapid analysis of small pharmaceutical compounds. Initially, SFC made its contributions in the preparative arena for chiral drugs but now has been applied to more general small molecule applications. For many separations, SFC can be superior to HPLC/UHPLC, especially in the speed of analysis. The phases used for SFC are different than those used for liquid chromatography so additional polar phases are required to exploit this technology.

The trend in the use of monoclonal antibodies and peptide-based compounds in drug development requires columns capable of providing high recovery separations of biologically derived compounds, oligonucleotides and biosimilars, both neat and in biological fluids. Column manufacturers are already responding with biocompatible columns that provide more selective separations with higher recovery.

1. SDi, Global Assessment Report, 2012-2016, (Los Angeles, October 2012).

Ron Majors
Ron Majors

About the Author
Ron Majors is a senior scientist consultant for Agilent Technologies.


blog comments powered by Disqus
LCGC E-mail Newsletters

Subscribe: Click to learn more about the newsletter
| Weekly
| Monthly
| Weekly

What role should the US government play in the current Ebola outbreak?
Finance development of drugs to treat/prevent disease.
Oversee medical treatment of patients in the US.
Provide treatment for patients globally.
All of the above.
No government involvement in patient treatment or drug development.
Finance development of drugs to treat/prevent disease.
Oversee medical treatment of patients in the US.
Provide treatment for patients globally.
All of the above.
No government involvement in patient treatment or drug development.
Jim Miller Outsourcing Outlook Jim MillerOutside Looking In
Cynthia Challener, PhD Ingredients Insider Cynthia ChallenerAdvances in Large-Scale Heterocyclic Synthesis
Jill Wechsler Regulatory Watch Jill Wechsler New Era for Generic Drugs
Sean Milmo European Regulatory WatchSean MilmoTackling Drug Shortages
New Congress to Tackle Health Reform, Biomedical Innovation, Tax Policy
Combination Products Challenge Biopharma Manufacturers
Seven Steps to Solving Tabletting and Tooling ProblemsStep 1: Clean
Legislators Urge Added Incentives for Ebola Drug Development
FDA Reorganization to Promote Drug Quality
Source: Pharmaceutical Technology Europe,
Click here