Book reviewers come in all stripes. They are generally perceived to be experts in the discipline that is the subject of the
book. Their extensive knowledge of the subject permits them to judge the correctness and depth of the information being imparted
by the author.
Industrial Bioseparations: Principles and Practice by Daniel Forciniti, Wiley-Blackwell, Oxford, UK, 2008, 309 pp., ISBN:
Daniel Forciniti conveys a great deal of information in an impressive work called Industrial Bioseparations. Given the breadth of this burgeoning field, few are qualified to review this book.
But the novice (such as myself) may perhaps also serve as a reviewer. He or she can attest to the author's helpful guidance,
to the principles that form the structure of the book, and to the aptness of the tools and practices the author uses to teach
the reader about the subject. In this sense, the reviewer's critique reflects his or her experience reading the book.
In the book's preface, Forciniti refers to the wealth of biotechnological advances that now produce therapeutic drugs and
other compounds. Because the end products are often proteins made from the genetically altered cells of organisms such as
bacteria, plants, and animals, they must be isolated from the fermentation mixes that produce them. The techniques required
for this purpose derive from biological operations that require engineering skills based on the physical sciences. These skills
have seldom, if ever, been dedicated to the resolution of such complexities. It should be emphasized that more than an elementary
knowledge of both the biological and physical sciences is required for this isolation, in a combination that translates into
an engineering application.
Forciniti undertakes and achieves his goal of teaching the principles and activities involved in bioseparations engineering
practices. He teaches in the definitive language of mathematics, and his approach to technical causes and effects will oblige
the purists who require certainties. Those secure in conclusions based on thermodynamics will applaud Forciniti's mathematical
logic. His language is impressively clear and exceptionally instructive and explanatory.
The book discusses the production and purification of biomacromolecular entities. It thoroughly explains solid– solid, solid–liquid,
and liquid–liquid separations. The principles that define the various states of matter are made plain in the book. The requisite
techniques for isolating and purifying the diverse products are considered and evaluated in abundant detail. The techniques
of flocculation, centrifugation, filtration, chromatography, and precipitation are investigated. Discussions of cell disruptions,
protein molecular structures, freeze-drying, osmosis, and reverse osmosis are not neglected.
The book also offers practical information that extends to pertinent materials and methods, even to pumps, valves, and mixers
as well as to separation cascades and types of chromatography and centrifugation. The breadth of Forciniti's coverage of technical
topics is awesome.
In dealing with solid–liquid separations, Forciniti discusses the one discipline with which the reviewer has some technical
familiarity (namely, filtration). The book discusses the materials and methods of conventional industrial filtrations dealing
with the plate-and-frame system and rotarydrum, vacuum-activated equipment. Forciniti also discusses unconventional filtrations
such as ultrafiltration and microfiltration in both the dead-end and cross-flow modes. He explains Darcy's Law both mathematically
and verbally in satisfying detail.
Forciniti is obviously familiar with the finer filtrations based on membranes in large-scale pharmaceutical manufacturing
and in making semiconductor chips. He cites appropriate sources and literature on the subject of filter manufacturing.
The book, necessarily limited at 309 pages, provides a surprising amount of operational details and imparts valuable information
to the reader. As Forciniti made evident in the preface to his book, he did not wish to design an encyclopedic work, but rather
a well-balanced book. He attained his goal with considerable distinction. This volume will serve as an invaluable introduction
and guide to those embarking on careers in the discipline of bioseparations. Foriciniti deserves our thanks for promoting
knowledge of this important endeavor.
Theodore H. Meltzer, PhD, is a principal at Capitola Consultancy, 8103 Hampden Ln.,
Bethesda, MD 20814-1244, tel. 301.986.8640, email@example.com