Changes in the biopharmaceutical industry have led to the need for new chromatographic techniques beyond traditional methods
such as hydrophobic interaction chromatography, (HIC), ion exchange chromatography, and affinity chromatography. Protein expression
titers and feedstock volumes have increased greatly during the past decade, and fermenter volumes of 12,000–15,000 L are not
"Some of the technologies that were developed about 30 years ago are no longer optimal for some of these new challenges,"
says Sylvio Bengio, scientific communications manager for Pall Life Sciences (East Hills, NY,
Conventional techniques, including the Protein A affinity, still work well. But the cost of these methods is weighing heavily
and has led to increasing interest in alternatives, especially new sorbents for mixed-mode chromatography and specially designed
Improving mixed-mode sorbents
In principle, mixed-mode chromatography is not new. However, researchers now are able to specifically design and finely "tune"
the hydrophobic dimension of the mixed-mode mechanism.
Converting a dilute feedstock to a high-purity protein typically involves three steps: a capture step, an intermediate step,
and a polishing step, which may involve ion exchange, but there are situations where this is no longer ideal. One example
is when processing large volumes of feedstream. Typically, to obtain sufficient capacity with conventional ion exchangers,
the conductivity of the feedstock must be adjusted by dilution, sometimes to as much as five times the original volume. Although
this practice is feasible at the laboratory scale involving 5- or 10-L volumes, working with a 10,000-L feedstream is another
challenge. "Obviously diluting three or four or five times is totally unacceptable because buffer storage volumes create problems,"
says Bengio. "Ideally, at the capture step, you would like to have a minimal dilution of the feedstock and still get good
capacity and recovery in one step; this is exactly what new mixed-mode platforms will do in most cases."
Monoclonals currently represent more than 35% of the proteins in clinical trials, and the current platform technology is affinity
chromatography on Protein A. Protein A sorbents are very selective for capturing antibodies from complex feedstreams with
high purity in just one step. One of the biggest problems with this ligand, however, is its cost. Some antibody processes
may require several hundred kilograms of pure mono clonal for different treatments. "There is strong pressure on the industry
to cut purification cost," says Bengio. "Chromatography and especially Protein A sorbents are one of the major contributors
to purification cost that the industry wants to cut."
Mixed-mode chromatography also has been used in place of or in combination with HIC processes (i.e., at the initial HIC step, in which case the methods would be orthogonal). Although conventional HIC is well-known, broadly
used, and effective at various stages in purification, it requires the addition of high concentrations of lyotropic salt,
typically ammonium sulfate, to the feedstock to promote protein binding to conventional HIC resin. Concentrations can range
from 1 to 3 M. At the laboratory or small scale, this is usually not a problem. "But in industry where there are thousands
of liters of feedstream, adding massive quantities of salt is a nightmare because salt is expensive and creates hardware problems.
Furthermore you have to recycle that salt for environmental protection purposes, and this increases the overall cost" says
Russell Jones, chromatography marketing manager at Pall Life Sciences, notes that a company using conventional HIC sorbents,
is likely to use several tons of lyotropic salt per year. "A process column containing hundreds of liters of conventional
HIC sorbent will require the use of significant volumes of buffers containing 1–3 M lyotropic salt, especially over the course
of an annual campaign of 30 cycles or more. This will not only have an impact on process cost but also on what you have to
do afterward to dispose of the salt in an environmentally friendly way."
Purification methods: old and new
Not only is Protein A expensive, but it leaches and must be removed. Furthermore, the Protein A resin is not stable to 1 M
NaOH, which is frequently used for cleaning. (Although GE Healthcare recently introduced "MabSelect SuRe," which is stable
in 0.5 M NaOH.) Also, the method requires elution at low pH, which tends to cause aggregation and damage the antibody.