Achieving Ultrafast Liquid Chromatography - Pharmaceutical Technology

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Achieving Ultrafast Liquid Chromatography
The authors review methods for reducing analysis time and increasing throughput that are reliable and maintain data integrity.


Pharmaceutical Technology


The downside to using a sub-2-μm-particle column is increased system pressure, which requires a limited column length to remain within the capabilities of the available hardware in the laboratory. This restriction occurs because the pressure arising from the column is in an inverse-square relationship to the particle diameter. Reducing the particle diameter by half results in a fourfold increase in observed pressure. This pressure relationship makes it difficult to use small-particle columns under a high (i.e., optimum) flow-rate range or choose a column long enough to achieve the desired resolution.

In response to this difficulty, some companies have developed specialized instruments with improved pressure tolerances. These instruments, however, have their own limitations when it comes to the accepted levels of performance expected from HPLC analysis with respect to injection reproducibility and gradient accuracy. In addition, these systems may have a higher cost of ownership because of the extreme conditions under which they operate and the resulting wear and tear on consumable components such as pump seals and injection-valve rotors. The question then arises: Is it really necessary or beneficial to use a sub 2-μm particle to increase the speed when the back pressure increases so much?

The authors suggest that using a well-packed column with uniform silica gel-based materials of a mid-size diameter is the proper solution (1). Mid-size particles are between 2 and 3 μm. Sub 2-μm particles are defined as small. This may seem like an academic difference, but recall the inverse-square relationship of particle size to pressure. Mid-size particles allow users to achieve high speed and high resolution simultaneously on existing equipment. Consider that the performance difference between a 2.2-μm column, for instance, and a 1.8-μm column is minimal, but the expected back pressure with the 2.2-μm particle column is less than two-thirds that of the sub-2-μm particle, meaning the highest performance level is achieved without sacrificing operability.

Furthermore, in some cases, small-particle columns, owing to their shorter length, may not be suitable for separating closely eluting peaks. If resolution is critical, the natural strategy is to use a longer column. With a small-particle column, it may be impossible to use a long column because the back pressure exceeds the allowed operating pressure, regardless of the system capability because every system has a limit. As a result, it is necessary to choose either a lower flow rate (thus eliminating the advantage of going to a smaller particle) or to switch to a larger particle size and longer column to reduce the back pressure.

The time needed to perform the separation is longer, so a balance must be struck between time and resolution. This problem demonstrates that one cannot necessarily have an ideal separation. The separation must be optimized for each individual's needs—the highest speed and the ultimate resolution may not always be possible in the same run. Columns with mid-size particles have proven to be optimal for balancing the time reduction and high resolution required at the highest levels. It is more useful if the same or better separation with a shorter run time can be achieved with lower back pressure.

Separation at elevated temperature

Interest in elevated-temperature liquid chromatography (LC) is increasing because better and faster separation can be obtained simultaneously by using higher temperatures. Separation performed at increased temperature has an effect similar to that of reducing particle size. Both techniques accelerate the mass diffusion of the analyte between the stationary phase and mobile phase, thus resulting in a "flatter" van Deemter curve.

Both factor A and factor C of the van Deemter equation decrease as temperature increases and result in a lower HETP. High-temperature HPLC also reduces the viscosity of the mobile phase, thus lowering the resistance to flow through the column and allowing greater flow rates compared with ambient conditions. The resistance to column flow is inversely proportional to temperature, such that the column pressure at 80 C is about 40% lower than that at 40 C. This relationship highlights the importance of temperature as a factor in accelerating separation. Of course, increasing the temperature of the column raises questions of itself.


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