Improved Mass Determination of Poly(ethylene glycols) by Electrospray Ion-Mobility Time-of-Flight Mass Spectrometry Coupled with Ion–Molecule Reactions - Pharmaceutical Technology

Latest Issue

Latest Issue
PharmTech Europe

Improved Mass Determination of Poly(ethylene glycols) by Electrospray Ion-Mobility Time-of-Flight Mass Spectrometry Coupled with Ion–Molecule Reactions
The authors developed a method to accurately measure the average molecular weight of large poly(ethylene glycols) (PEGs) using ion-mobility time-of-flight mass spectrometry coupled with gas-phase ion–molecule reactions.

Pharmaceutical Technology


1. J.M. Harris, Introduction to Biotechnical and Biomedical Applications of Poly(Ethylene Glycol) in Poly(Ethylene Glycol) Chemistry: Biotechnical and Biomedical Applications (Topics in Applied Chemistry), J.M. Harris, Ed. (Plenum, New York, 1992).

2. A. Kozlowski and J. M. Harris, "Improvements in Protein PEGylation: Pegylated Interferons for Treatment of Hepatitis C," J. Controlled Release 72, 217–224 (2001).

3. D.J. Winzor. "Analytical Exclusion Chromatography," J. Biochem. Biophys. Methods 56 (1–3), 15–52 (2003).

4. J.Y. Shey and C.M. Sun, "Liquid-Phase Combinatorial Reaction Monitoring by Conventional 1H NMR Spectroscopy," Tetrahedron Lett. 43, 1725–1729 (2002).

5. J.L. Koenig and A.C. Angood, "Raman Spectra of Poly(ethylene glycols) in Solution," J. Polymer Sci. Part A-2: Polymer Physics 8 (10), 1787–1796 (1970).

6. P.B. O'Connor and F.W. McLafferty, "Oligomer Characterization of 4-23 kDa Polymers by Electrospray Fourier Transform Mass Spectrometry," J. Am. Chem. Soc. 117, 12826–12831 (1995).

7. S.D. Hanton, "Mass Spectrometry of Polymers and Polymer Surfaces," Chem. Rev. 101 (2), 527–570 (2001).

8. C.G. de Koster et al. "Endgroup analysis of Polyethylene Glycol Polymers by Matrix-Assisted Laser Desorption/Ionization Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry," Rapid Commun. Mass Spectrom. 9 (10), 957–962 (1995).

9. L.M. Nuwaysir, C.L. Wilkins, and W.J. Simonsick, Jr., "Analysis of Copolymers by Laser Desorption Fourier Transform Mass Spectrometry," J. Am. Soc. Mass Spectrom. 1, 66–71 (1990).

10. E.P. Maziarz, III, G.A. Baker, and T.D. Wood. "Capitalizing on the High Mass Accuracy of Electrospray Ionization Fourier Transform Mass Spectrometry for Synthetic Polymer Characterization: A Detailed Investigation of Poly(dimethylsiloxane)," Macromolecules 32, 4411–4418 (1999).

11. M.W.F. Nielen, "MALDI Time-of-Flight Mass Spectrometry of Synthetic Polymers," Mass Spectrom. Rev. 18, 309–344 (1999).

12. T. Nohmi and J.B. Fenn "Electrospray Mass Spectrometry of Poly(ethy1ene glycols) with Molecular Weights up to Five Million" J. Am. Chem. Soc. 114, 3241–3246 (1992).

13. J.J. Palmgred et al.,"Liquid Chromatographic–Electrospray Ionization Mass Spectrometric Analysis of Neutral and Charged Polyethylene Glycols," J. Chromatogr. A 976, 165–170 (2002).

14. J.D. Lennon, S.P. Cole, and G.L. Glish, "Ion–Molecule Reactions To Chemically Deconvolute the Electrospray Ionization MassSpectra of Synthetic Polymers," Anal. Chem. 78, 8472–8476 (2006).

15. D. Bagal, H. Zhang, and P.D. Schnier, "Gas-Phase Proton-Transfer Chemistry Coupled with TOF Mass Spectrometry and Ion Mobility-MS for the Facile Analysis of Poly(ethylene glycols) and PEGylated Polypeptide Conjugates," Anal. Chem. 80, 2408–2418 (2008).

16. J.L. Stephenson and S.A. McLuckey, "Reactions of Poly(ethylene glycol) Cations with Iodide and Perfluorocarbon Anions," J. Am. Soc. Mass Spectrom. 9, 957-9651, (1998).

17. S. D. Pringle et al., "An Investigation of the Mobility Separation of Some Peptide and Protein Ions Using a New Hybrid Quadrupole/Travelling Wave IMS/oa-TOF Instrument." Int. J. Mass Spectrom. 261, 1–12 (2007).

18. K. Giles et al., "Applications of a travelling Wave-Based Radio-Frequency Only Stacked Ring Ion Guide," Rapid Commun. Mass Spectrom. 18, 2401–2414 (2004).

Asish B. Chakraborty is a senior scientist, Weibin Chen*, is a principal research chemist, and John C. Gebler is the director, all at Biopharmaceutical Sciences, Waters Corporation, 34 Maple Street, Milford, MA 01757,

*To whom all correspondence should be addressed.

What would you do differently? Email your thoughts about this paper to
and we may post them to the site.


blog comments powered by Disqus
LCGC E-mail Newsletters

Subscribe: Click to learn more about the newsletter
| Weekly
| Monthly
| Weekly

Which of the following business challenge poses the greatest threat to your company?
Building a sustainable pipeline of products
Attracting a skilled workforce
Obtaining/maintaining adequate financing
Regulatory compliance
Building a sustainable pipeline of products
Attracting a skilled workforce
Obtaining/maintaining adequate financing
Regulatory compliance
View Results
Eric Langer Outsourcing Outlook Eric LangerBiopharma Outsourcing Activities Update
Cynthia Challener, PhD Ingredients Insider Cynthia Challener, PhDAppropriate Process Design Critical for Commercial Manufacture of Highly Potent APIs
Jill Wechsler Regulatory Watch Jill Wechsler FDA and Manufacturers Seek a More Secure Drug Supply Chain
Sean Milmo European Regulatory WatcchSean MilmoQuality by Design?Bridging the Gap between Concept and Implementation
Medicare Payment Data Raises Questions About Drug Costs
FDA Wants You!
A New Strategy to Tackle Antibiotic Resistance
Drug-Diagnostic Development Stymied by Payer Concerns
Obama Administration Halts Attack on Medicare Drug Plans
Source: Pharmaceutical Technology,
Click here