Industrial Applications of Whole-Cell Biocatalysis - Pharmaceutical Technology

Latest Issue

Latest Issue
PharmTech Europe

Industrial Applications of Whole-Cell Biocatalysis
Recombinant microbial whole-cell biocatalysis is a valuable approach for producing enantiomerically pure interemediates. The authors examine several groups of enzymes using this approach: dehydrogenases, hydantoinases, and acylases.

Pharmaceutical Technology

7. F. Cecere et al., "D-Carbamylamino Acids and Corresponding D-Amino Acids," Ger. Offen, 2,621, 076, Addn. to Ger. Offen. 2,422,737, Dec. 1976.

8. M. Kato, H. Kitagawa, and T. Miyoshi, "Microbial Racemization of Optically Active 5-Substituted Hydantions," Japanese Patent 62122591, 1987.

9. M. Pietzsch, C. Syldatk, and F. Wagner, "Isolation and Characterization of a New, Nonpyrioxal-5'-Phosphate Dependent Hydantion Racemase," DECHEMA Biotechnol. Conf. 4 (Pt A. Lect), 259–262 (1990).

10. C. Syldatk et al., "Microbial Hydantoinases—Industrial Enzymes from the Origin of Life?," Appl. Microbiol. Biotechnol. 51 (3), 293–309 (1999).

11. C. Gross, C. Syldatk, and F. Wagner, "Screening Method for Microorganisms Producing L-Amino Acids from D,L-5-Monosubstituted Hydantoins," Biotechnol. Tech. 1 (2) 85–90 (1987).

12. Y. Nishida et al., "Enzymatic Production of L-tryptophan from D,L-5-Indolylmethylhydantoin by Flavobacterium sp.," Enzyme Microb. Technol. 9 (12), 721–725 (1987).

13. D. Cotoras and F. Wagner, "Stereospecific Hydrolysis of 5-Monosubstituted Hydantoins," 3rd Eur. Congr. Biotechnol. 1, 351–356 (1984).

14. F. Wagner et al., "Microorganisms Producing L- Amino Acids, " German Patent DE 19519717, 1996

15. T. Wagner, B. Hantke, and F.J. Wagner, "Production of L-methionine from D,L-5-(2-methylthioethyl) Hydantoin by Resting Cells of a New Mutant Strain of Arthrobacter species DSM 7330," J. Biotechnol. 46 (1), 63–68 (1996).

16. O. May et al., "Substrate-Dependent Enantioselectivity of a Novel Hydantoinase from Arthrobacter aurescens DSM 3745: Purification and Characterization as New Member of Cyclic Amidases," J. Biotechnol. 61 (1) 1–13 (1998).

17. D. Volkel and F. Wagner, "Reaction Mechanism for the Conversion of 5-Monosubstituted Hydantoins to Enantiomerically Pure L-Amino Acids," Ann. N.Y. Acad. Sci., 750, 1–9 (1995).

18. O. May, P.T. Nguyen, and F.H. Arnold, "Inverting Enantioselectivity by Directed Evolution of Hydantoinase for Improved Production of L-Methionine," Nat. Biotechnol. 18 (3), 317–320 (2000).

19. A.S. Bommarius et al., "Operational Stability of Enzymes. Acylase-Catalyzed Resolution of N-Acetyl Amino Acids to Enantiomerically Pure L-Amino Acids," Ann. N. Y. Acad. Sci. 672, 126–136 (1992).

20. A.S. Bommarius, M. Schwarm, and K. Drauz, "The Membrane Reactor for the Acylase Process—From Laboratory Results to Commercial Scale," Chimica Oggi. 14 (10), 61–64 (1996).


blog comments powered by Disqus
LCGC E-mail Newsletters

Subscribe: Click to learn more about the newsletter
| Weekly
| Monthly
| Weekly

Which of the following business challenge poses the greatest threat to your company?
Building a sustainable pipeline of products
Attracting a skilled workforce
Obtaining/maintaining adequate financing
Regulatory compliance
Building a sustainable pipeline of products
Attracting a skilled workforce
Obtaining/maintaining adequate financing
Regulatory compliance
View Results
Eric Langer Outsourcing Outlook Eric LangerBiopharma Outsourcing Activities Update
Cynthia Challener, PhD Ingredients Insider Cynthia Challener, PhDAppropriate Process Design Critical for Commercial Manufacture of Highly Potent APIs
Jill Wechsler Regulatory Watch Jill Wechsler FDA and Manufacturers Seek a More Secure Drug Supply Chain
Sean Milmo European Regulatory WatcchSean MilmoQuality by Design?Bridging the Gap between Concept and Implementation
Medicare Payment Data Raises Questions About Drug Costs
FDA Wants You!
A New Strategy to Tackle Antibiotic Resistance
Drug-Diagnostic Development Stymied by Payer Concerns
Obama Administration Halts Attack on Medicare Drug Plans
Source: Pharmaceutical Technology,
Click here