Developing a modified Pulsincap system - Pharmaceutical Technology

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PharmTech Europe

Developing a modified Pulsincap system

Pharmaceutical Technology Europe
Volume 21, Issue 11

Evaluation of prepared microcapsules

Determination of drug entrapment efficiency

The efficiency of drug entrapment8 for each batch was calculated in terms of percentage drug entrapment (PDE) using the formula in Equation 2.

The author says...
Theoretical drug loading was calculated on the assumption that all of the drug in the polymer solution used was entrapped in the microcapsules and that none was lost during the preparation of the microcapsules.

Practical drug loading was determined by taking accurately weighed microcapsules equivalent to 100 mg of drug and crushed using a glass pestle and mortar. Methanol was added in small quantities to the mortar and transferred to a 50mL volumetric flask. The mortar was washed with methanol to remove all traces of drug and transferred to the flask. The volume was then increased to 50 mL with the same solvent. The flasks were kept on a sonicator for 5 min. The solution was then filtered using a Whatman filter paper and a suitable dilution was made. Absorbance was read at 281 nm against methanol as a blank. The PDEs of DM1 to DM4 are reported in Table 1.

Micromeritic properties of prepared microcapsules

Table 1: Physical characteristics of DS microcapsules.
The micromeritic properties of the microcapsules9 were determined by the angle of repose (using the fixedbase funnel method), bulk density, tapped density and Carr's index. Bulk and tapped densities were measured in 10 mL of a graduated glass cylinder. The sample contained in the cylinder was tapped mechanically by a constantvelocity rotating cam. The results are shown in Table 1.

Particle size and morphology study of microcapsules

Figure 1
The average particle size and morphology of the drugloaded microcapsules10 (n=100) of batches DM1 to DM4 were assessed by using a digital optical microscope (Labomed CX RIII, Ambala, India) and SEM microscopy, respectively. The microcapsules' morphology was characterized by their sphericity and clumping. The results are reported in Table 1 and Figure 1.


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