Analytical Applications - Pharmaceutical Technology

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PharmTech Europe

Analytical Applications
Several industry experts describe applications in pharmaceutical applications, including on-line total organic carbon analysis, ultra-fast liquid chromatography, rapid microbial testing, and differential scanning calorimetry-Raman Spectroscopy.


Pharmaceutical Technology
Volume 34, pp. s36-s30


Figure 1 (TOC): A 30-day measurement-system equivalency test of off-line and on-line total organic carbon (TOC) analysis. (FIGURE 1 (TOC) IS COURTESY OF GE Analytical Instruments)
Figure 1 (TOC) is an example of a 30-day measurement-system equivalancy test protocol from a pharmaceutical company. In this protocol, the statistical parmameters of mean and standard deviation represent data-quality equivalency. The mean is determined from repeated measurements of the process. This average includes contributions from the pharmaceutical water itself, the sampling process, and the inherent accuracy of the measurement device. By pairing the on-line and laboratory data collection, the process water variability remains common between the two systems. In addition, the inherent accuracy of the instruments remains common, based on previous method validation results. The mean serves to quantify the added TOC contributions of the sampling process itself. Additionally, the standard deviation is used to assess the repeatability of the measurement system, taking into account the variation of the sampling process and the measurement device.

TOC section reference

1. FDA, Guidance for Industry: PAT (Rockville, MD, 2004).

Ultra-fast liquid chromatography

Simon Robinson, HPLC product manager, Shimadzu Scientific Instruments (Columbia, MD)

Over-the-counter cold medicines often contain multiple active ingredients. These actives include combinations of decongestants, antihistamines, pain relievers, cough suppressants, and expectorants in addition to numerous vitamins and herbal extracts—all of which exhibit different chemical properties. A wide range of analyte polarities often makes developing chromatographic methods challenging. Separating ingredients in a cold medicine is important to understand the content of each medication and product quality. A high-speed liquid chromatographic system (Prominence UFLCxr with a SPD-M20A photodiode array detector, Shimazdu Scientific Instruments) was used to analyzed 20 common cold medcines.


Figure 1 (UFLC): Results of five serial ultra-high-speed liquid chromatographic analyses. (FIGURE 1 (UFLC) IS COURTESY OF SHIMAZDU SCIENTIFIC INSTRUMENTS)
Methods. A standard mixture of cold-medicine ingredients was prepared (dissolving them in Mobile Phase A/acetonitrile = 1/1 (v/v), 100 mg/L, each) and analyzed using the conditions shown in Table I (UF LC). The mixture consisted of thiamine, acetaminophen, caffeine, riboflavin, hesperidin, ethenzamide, chlorpheniramine, ambroxol, noscapine, isopropamide, ispropylantipyrine, dextromethorphan, glycyrrhizin, bromhexine, clemastine, and ibruprofen. The contents of a capsule of cold medicine was dissolved in 100 mL of Mobile Phase A/acetonitrile = 1/1 (v/v), or in the case of tablet form, a single tablet was dissolved in 50 mL of the previously described mobile-phase acetonitrile solution. The resultant solution was passed through a 0.22 m pore membrane filter and analyzed using the conditions in Table I (UFLC).


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