Investigating the Influence of Glycosylation on Protein Conformation and Dynamics - Pharmaceutical Technology

Latest Issue
PharmTech

Latest Issue
PharmTech Europe

Investigating the Influence of Glycosylation on Protein Conformation and Dynamics
The authors review and discuss the influence of glycans on the conformation of a representative IgG1 biopharmceutical using H/DX-MS as an analytical tool.


Pharmaceutical Technology
Volume 34, pp. s12-s17

Acknowledgments

The authors would like to thank Dr. Helena Madden, Dr. Rohin Mhatre, and Dr. John R. Engen for helpful discussions and for critical reading of the manuscript.

Damian Houde* is a scientist, and Steven A. Berkowitz, is a principal investigator, both at Biogen Idec, Inc., 14 Cambridge Center, Cambridge, MA 02142, tel: 617.914.5841, fax: 617.679.3476.

*To whom all correspondence should be addressed.

References

1. C.T. Walsh, S. Garneau–Tsodikova, and G. J. Gatto Jr., Angew Che m.Int. Ed. Engl. 44 (45), 7342–7372 (2005).

2. G. Walsh and R. Jefferis, Nat. Biotechnol. 24 (10) ,1241–1252 (2006).

3. J.N. Arnold et al.,. Annu. Rev. Immunol. 25, 21–50 (2007).

4. N.Mitra et al., Trends Biochem. Sci. 31 (3), 156–163 (2006).

5. R.J. Sola, J.A. Rodriguez–Martinez, and K. Griebenow, Cell. Mol. Life Sci. 64 (16) 2133–2152 (2007).

6. T.W. Rademacher, R.B. Parekh, and R.A. Dwek, Annu. Rev, Biochem, 57, 785–838 (1988).

7. P. Van den Steen et al., Crit. Rev. Biochem. Mol. Biol. 33 (3), 151–208 (1998).

8. N.J. Agard and C.R. Bertozzi, Acc. Chem. Res. 42 (6), 788–797 (2009).

9. J. Zaia, Chem. Biol. 15 (9) 881–892 (2008).

10. M.R. Wormald and R.A. Dwek, Structure 7 (7), R155–160 (1999).

11. J.J. Caramelo and A.J. Parodi, Semin. Cell. Dev. Biol. 18 (6), 732–742 (2007).

12. J.R. Engen, Anal. Chem. 81 (19), 7870–7875 (2009).

13. A. Hvidt and K. Linderstrom–Lang, Biochim. Biophys. Acta 14 (4) 574–575 (1954).

14. P.I. Haris and D. Chapman, Biopolymers 37 (4), 251–263 (1995).


ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters

Subscribe: Click to learn more about the newsletter
| Weekly
| Monthly
|Monthly
| Weekly

Survey
Which of the following business challenge poses the greatest threat to your company?
Building a sustainable pipeline of products
Attracting a skilled workforce
Obtaining/maintaining adequate financing
Regulatory compliance
Building a sustainable pipeline of products
27%
Attracting a skilled workforce
30%
Obtaining/maintaining adequate financing
10%
Regulatory compliance
33%
View Results
Eric Langer Outsourcing Outlook Eric LangerBiopharma Outsourcing Activities Update
Cynthia Challener, PhD Ingredients Insider Cynthia Challener, PhDAppropriate Process Design Critical for Commercial Manufacture of Highly Potent APIs
Jill Wechsler Regulatory Watch Jill Wechsler FDA and Manufacturers Seek a More Secure Drug Supply Chain
Sean Milmo European Regulatory WatcchSean MilmoQuality by Design?Bridging the Gap between Concept and Implementation
Medicare Payment Data Raises Questions About Drug Costs
FDA Wants You!
A New Strategy to Tackle Antibiotic Resistance
Drug-Diagnostic Development Stymied by Payer Concerns
Obama Administration Halts Attack on Medicare Drug Plans
Source: Pharmaceutical Technology,
Click here