Investigating the Influence of Glycosylation on Protein Conformation and Dynamics - Pharmaceutical Technology

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Investigating the Influence of Glycosylation on Protein Conformation and Dynamics
The authors review and discuss the influence of glycans on the conformation of a representative IgG1 biopharmceutical using H/DX-MS as an analytical tool.

Pharmaceutical Technology
Volume 34, pp. s12-s17


The authors would like to thank Dr. Helena Madden, Dr. Rohin Mhatre, and Dr. John R. Engen for helpful discussions and for critical reading of the manuscript.

Damian Houde* is a scientist, and Steven A. Berkowitz, is a principal investigator, both at Biogen Idec, Inc., 14 Cambridge Center, Cambridge, MA 02142, tel: 617.914.5841, fax: 617.679.3476.

*To whom all correspondence should be addressed.


1. C.T. Walsh, S. Garneau–Tsodikova, and G. J. Gatto Jr., Angew Che m.Int. Ed. Engl. 44 (45), 7342–7372 (2005).

2. G. Walsh and R. Jefferis, Nat. Biotechnol. 24 (10) ,1241–1252 (2006).

3. J.N. Arnold et al.,. Annu. Rev. Immunol. 25, 21–50 (2007).

4. N.Mitra et al., Trends Biochem. Sci. 31 (3), 156–163 (2006).

5. R.J. Sola, J.A. Rodriguez–Martinez, and K. Griebenow, Cell. Mol. Life Sci. 64 (16) 2133–2152 (2007).

6. T.W. Rademacher, R.B. Parekh, and R.A. Dwek, Annu. Rev, Biochem, 57, 785–838 (1988).

7. P. Van den Steen et al., Crit. Rev. Biochem. Mol. Biol. 33 (3), 151–208 (1998).

8. N.J. Agard and C.R. Bertozzi, Acc. Chem. Res. 42 (6), 788–797 (2009).

9. J. Zaia, Chem. Biol. 15 (9) 881–892 (2008).

10. M.R. Wormald and R.A. Dwek, Structure 7 (7), R155–160 (1999).

11. J.J. Caramelo and A.J. Parodi, Semin. Cell. Dev. Biol. 18 (6), 732–742 (2007).

12. J.R. Engen, Anal. Chem. 81 (19), 7870–7875 (2009).

13. A. Hvidt and K. Linderstrom–Lang, Biochim. Biophys. Acta 14 (4) 574–575 (1954).

14. P.I. Haris and D. Chapman, Biopolymers 37 (4), 251–263 (1995).


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