Adsorption-Free UHPLC

Published on: 
Pharmaceutical Technology, Pharmaceutical Technology, September 2022, Volume 46, Issue 9
Pages: 12

Shimadzu’s Nexera XS inert is designed to prevent both adsorption of target molecules to metal surfaces and mobile-phase corrosion.

Shimadzu’s Nexera XS inert is a bioinert and biocompatible ultra-high performance liquid chromatography that is designed to prevent the adsorption of target molecules to metal surfaces and mobile-phase corrosion caused by high salt content and extreme pH conditions.

The system utilizes a metalfree sample flow path, which prevents unwanted interaction with biopolymers that are prone to adsorption onto exposed metal sites, resulting in reduced sample loss and excellent peak shape in data results. Additionally, all wetted surfaces of the system use corrosionresistant materials, which are designed to promote stability against mobile-phase solvents that contain high concentrations of salts or acids. This makes it well-suited for analyzing biopharmaceutical molecules, such as antibodies, peptides, and nucleic acid drugs.

The system uses a stainless-steel-casing-reinforced polyether ether ketone tubing to promote mechanical and chemical resistance. It is designed for a pressure tolerance of >100 MPa while maintaining a bioinert flow path. An optional pHM-40 offers realtime measurement of the mobile-phase pH during an experiment.