New England Biolabs Luna Probe One-Step RT-qPCR Mix with UDG enables sensitive, linear, real-time detection of target RNA sequences.
New England Biolabs (NEB) Luna Probe One-Step RT- quantitative polymerase chain reaction (qPCR) Mix with uracil-DNA glycosylase (UDG) enables sensitive, linear, real-time detection of target RNA sequences in a room temperature-stable format. When measured in NEB’s “dot in boxes” scoring method—a high-throughput data visualization tool that provides quality scores for qPCR outcomes—the LyoPrime Luna outperformed many competitor products and strongly matched the company’s liquid mix, according to the company.
The lyophilized mix contains murine RNase Inhibitor, deoxynucleoside triphosphate, and a passive reference dye. It incorporates thermolabile UDG, which is well-suited to RNA workflows, as well as deoxyuridine triphosphate to reduce carryover contamination risk between reactions.