Ultra-Low Sample Optimized Mass Spectrometer

Published on: 
Pharmaceutical Technology, Pharmaceutical Technology, July 2023, Volume 47, Issue 7
Pages: 12

Bruker Corporations timsTOF Ultra mass spectrometer features a captive spray ionization ino sources that provides optimized vortex gas flow.

Bruker’s timsTOF Ultra mass spectrometer is designed for use with the company’s 4D-proteomics timsTOF platform. The device uses a new captive spray ionization ion source that provides a larger capillary relative to past models as well as optimized vortex gas flow.

The device is designed to identify over 55,000 peptides that map into 5000 protein groups at the single-cell level of 0.125 nanogram protein loading at less than 1% false discovery rate. More than 4800 protein groups are quantified at a coefficient of variation of less than 20%.

The parallel accumulation-serial fragmentation duty cycle can go as fast as 300 Hz for tandem mass spectrometry, which is intended to provide performance improvements for processes involving ultra-low sample amounts. This includes unbiased single-cell proteomics and single-cell lipidomics, unbiased spatial proteomics, immunopeptidomics, phosphoproteomics, post-translational modification analysis, and protein–protein interaction studies.