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The authors explain chemical transformations that are achievable through certain biocatalytic routes.
The pharmaceutical industry is under severe pressure to make its API processes greener, lower costs, minimize waste, and shorten existing syntheses. The need for economical, robust, scalable, and reliable processes for the synthesis of chiral APIs and intermediates has resulted in process chemists tuning their skills at the interface of chemistry and biology and embracing biocatalysts and biocatalytic processes in organic synthesis. This shift has resulted in biocatalysis becoming the workhorse of the chemists' toolbox for chiral chemistry.
Why has there been a surge in the application of this green technology? The key difference in using biocatalysis today compared with 10 years ago is that now all the supporting technologies that can make a difference in enzyme development, such as bioinformatics, enzyme evolution, and high-throughput screening, allow for an efficient turnaround in process development and enzyme evolution. Libraries of enzymes in the form of screening panels that contain all the materials required to screen against a given substrate can be used as an efficient way to identify and use biocatalysts for a given process. For example, the biocatalysis group at Almac has developed the selectAZyme platform, a proprietary platform that consists of a range of enzyme types, including hydrolases, carbonyl reductases, transaminases, P450 monooxygenases, nitrile hydratases, and nitrilases, to provide access to a broad range of chiral compounds, such as acids, esters, alcohols, amines, and amides. Figure 1 illustrates typical transformations performed by the selectAZyme platform.
FIgure 1: Possible transformations using biocatalysis (selectAZyme platform, Almac).
The timeline for selection of a given biocatalyst, scale-up, and manufacture of a given product is similar to that of the timelines for conventional chemistry optimization and scale-up. Typical timelines are shown in Figure 2.
Figure 2: Example of a timeline for implementation of an enzyme platform (selectAZyme, Almac) involving enzyme selection, scale-up, and commercial manufacture.
To illustrate the improvements in a synthesis that can be made using biocatalysis, take the example of Phase IIb compound in which nine steps of chemistry resulted in the formation of three chiral centers of a registered starting material with a global yield of 7.4%. The project was initiated with certain key objectives:
The original chemistry involved a myriad of steps, including a late-stage classical resolution using an expensive amine resolving agent and high-pressure hydrogenation using metal catalysis. The late-stage resolution resulted in large volumes having to be processed to step seven in the original synthesis. Almac's challenge was to make a scalable lower-volume route that had green and cost incentives for changing the process. Almac completed route invention, proof-of-concept demonstration, scale-up of hundreds of kilograms, and final commercial production of metric tons. The revised route consisted of five steps using three different selectAZyme enzymes with a global yield of 23.4%.
Innovation was achieved by delivering a green process that was scalable, derived from readily available feedstocks, and used off-the-shelf selectAZyme enzymes. From retrosynthetic analysis, it was shown that the registered starting material could be made from feedstocks that would not have any long-term supply issues and could be sourced readily from India and China. Having the proposed route on paper, the next step was to synthesize the key intermediates and begin enzyme screening.
Plant focus: High-potency manufacturing and sustainability at Pfizer
The project involved an early-stage bioresolution that resulted in a carboxylic acid product with > 96% enantiomeric excess (ee). From this point, a bioreduction step introduced another chiral center. Key to this enzyme screening was to find a carbonyl reductase (CRED) enzyme that was able to stereospecifically reduce the ketone of the desired enantiomer feedstock and not the undesired (2% ee) enantiomer from the bioresolution step. The CRED identified resulted in a stereospecific reduction and subsequent biopolish of the diastereomeric mixture. The remaining undesired ketone was easily removed using conventional work-up at the next step. The process ran from start to finish using two solvent combinations. Having developed the process, all stereoisomers (seven different products) were synthesized readily from other key selectAZyme enzymes, so analytical development could be undertaken to determine the fate of these potential impurities. The summarized advantages of the green enzyme process are shown in Table I.
Table I: Comparison of process conditions and efficiencies for a selected synthesis when made from a chemical route and a biocatalytic route.
It is clear from the example described herein that biocatalysis offers an attractive approach for a synthesis, which can result in greener processes and lower API costs. Advances in evolution technologies and metagenomic programs help to further enhance biocatalysis as a tool in chemical syntheses. Biocatalysis is a maturing technology and aids in the supply and delivery of chiral intermediates, fine chemicals, and APIs.
Tom Moody, PhD,* is head of biocatalysis and isotope chemistry, and Gareth Brown, PhD, is biocatalysis senior chemist, both at Almac, Stranmillis Road, Belfast, Northern Ireland, BT95AG, firstname.lastname@example.org
*To whom correspondence should be addressed.