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Pharmaceutical Technology's In the Lab eNewsletter
The LenS3 Multi-Angle Light Scattering (MALS) detector, presented at Pittcon 2019 in Philadelphia, PA, uses a new approach for measuring molecular weight and radius of gyration of synthetic polymers, polysaccharides, proteins, and biopolymers.
Tosoh Bioscience’s LenS3 Multi-Angle Light Scattering (MALS) detector offers a new approach for measuring molecular weight (MW) and radius of gyration (Rg) of synthetic polymers, polysaccharides, proteins, and biopolymers.
The new detector has a new optical design, cell-block assembly, and calculation methodology. Featuring a patent-pending cell geometry and a lower wavelength laser (at 514 nm), the detector allows direct measurement of MW and provides high sensitivity. Additionally, with an improved normalization process and a new method for using the angular dependence, the determination of radius of gyration of smaller macromolecules below 10 nm in gyration size Rgis now possible, according to the company.
“Light scattering of macromolecules in solution has seen increasing interest over the past decades from scientists studying polymers and proteins,” said Tosoh Bioscience in a March 19, 2019 press release. “[High-performance liquid chromatography–size-exclusion chromatography] (HPLC-SEC) systems, coupled with online light-scattering detection, have proven to be a powerful tool for measuring absolute molecular weight and the gyration size of the macromolecules. The technique is also essential in identification and quantification of aggregation levels in proteins and monoclonal antibodies and in studying their conformation in various solution conditions."
The instrument is supported by SECview software, which streamlines the calculations required by the advanced detectors and performs analysis of sample-of-interest using multiple calibration methods simultaneously. The software is capable of gel permeation chromatography system/hardware control, multi-channel data acquisition, data processing, and analysis.
Available for sale in the Americas, the detector was introduced at Pittcon 2019 in Philadelphia, PA, from March 17–21, 2019.
Source: Tosoh Bioscience