Achieve Detection of Tailless mRNA with Capillary Electrophoresis

Join us to explore tailless mRNA detection with the BioPhase 8800 system and the RNA 9000 Purity & Integrity kit. Enhance mRNA therapeutics with advanced methods.

Register Free: https://www.chromatographyonline.com/lcgc_w/tailless-mrna

Event Overview:

As mRNA-based therapeutics continue to advance, there is an increasing need for high-resolution analytical methods capable of distinguishing RNA strands of similar length. One critical quality attribute (CQA), highlighted in the USP draft chapter on analytical procedures for mRNA vaccines, is the assessment of the percentage of transcripts lacking a 3’ poly(A) tail. The draft chapter recommends ribonuclease H-mediated targeted cleavage followed by ion-pair reverse-phase HPLC (IP-RP-HPLC) as a suitable technology to assess this CQA. An alternative approach is capillary gel electrophoresis (CGE), which is well established as an analytical technology in the mRNA field to assess mRNA fragmentation and integrity.

Join us for a webinar as we discuss how to resolve tailless mRNA from full length transcripts using the BioPhase 8800 system and the RNA 9000 Purity & Integrity kit. Learn how you can assess mRNA integrity and quantify mRNA impurities to a high degree of accuracy using the power of multicapillary electrophoresis.

Key Learning Objectives:

• Understand the analytical challenges in detecting tailless mRNA species
• Explore how to use multicapillary electrophoresis to resolve similar-length mRNA species
• Learn how to detect mRNA impurities using GCE and its resolution capabilities

Who Should Attend:

• Directors of R&D
• Senior research scientists
• Chief technical officers
• Principal scientist
• Technology directors

Speaker:

Ryan Williams
Senior Scientist - Analytics
Vernal Biosciences

Ryan Williams is a senior analytical scientist at Vernal Biosciences. With over a decade of biomolecule experience in ISO-compliant environments, he excels in purification and bioanalytical method development. He focuses on mRNA analytics and detecting challenging impurities. Williams holds a BS in biology from Norwich University.

Register Free: https://www.chromatographyonline.com/lcgc_w/tailless-mrna