Shimadzu, one of the leading manufacturers in analytical instrumentation, and the Molnar Institute in Berlin, Germany, cooperate in applying DryLab2010 software and the new generation of Shimadzu UHPLC instruments.
Method registration and validation requires detailed results concerning stability and robustness according to a design space. The well-known and accepted DryLab* software offers unique tools for supporting method development. Based on defined experiments, the design space of the method is modeled in a 3D cube. Each side represents a combination of parameters (mobile phase composition, temperature and pH) – moving inside this cube, the associated separation is modeled and displayed. Areas with good separation are displayed to visualize the design space of this method.
The accuracy of these calculations has been proven by many users. Prediction of retention, using DryLab’s algorithms, matches very well with real results. The design space model adds real value by providing a framework for small method adaptation, which makes it easier to adapt parameters based on new columns, detection or instrumentation as long these changes are executed within the given ‘space’.
Shimadzu’s UHPLC system is controlled via a software interface to LC solution software, methods can be set in PeakMatch and data files are read directly.
(*DryLab is a product of the Molnár Institute of applied chromatography Berlin)
For further details please visit www.shimadzu.eu