Richer Fragmentation Coverage for Faster and More Confident Metabolite Identification

Discover how electron activated dissociation (EAD) delivers structurally diagnostic fragments for faster, more confident metabolite identification.

Register Free: https://www.pharmtech.com/pt_w/metabolite-identification

Event Overview:

Confident metabolite identification depends on more than detection. Drug metabolism teams must localize the exact site of metabolism to guide medicinal chemistry decisions. Collision-induced dissociation (CID) often provides limited structural information, particularly for labile phase II conjugates such as glucuronides, which readily undergo neutral loss and leave the modification site ambiguous. Electron activated dissociation (EAD) on the ZenoTOF systems provides complementary fragmentation that preserves labile modifications and generates diagnostic ions, which can tell apart isomeric metabolites such as N- and O-glucuronides. Zeno trap enhanced MS/MS sensitivity and combined CID/EAD spectra speed up confident structure assignment for low level metabolites.

Key Learning Objectives:

• Leverage EAD to retain labile conjugates and yield diagnostic fragment ions
• Differentiate isomeric metabolites such as N- versus O-glucuronides
• Accelerate confident metabolite structure assignment

Who Should Attend:

• Drug metabolism and biotransformation scientists
• Bioanalytical scientists
• DMPK lab managers and directors
• Medicinal chemists
• CRO scientists
• Mass spectrometry core facility staff

Speaker:

Eshani Galermo, PhD
Staff Scientist, Global Pharma & Biopharma Applications
SCIEX

Eshani Galermo, PhD holds a doctorate degree from the University of California, Davis in Analytical Chemistry, where she developed mass spectrometry based methods to study carbohydrate structures and functions in gut health. Following her academic years, Eshani was part of biopharmaceutical and CRO companies where she led LC-MS method development for bioanalysis of small and large molecules. Eshani has been with SCIEX for six years now and is currently a Staff Scientist at SCIEX. She focuses on providing solutions to analytical challenges for biopharma quantitation and pharma applications by employing the latest mass spectrometry technologies.

Register Free: https://www.pharmtech.com/pt_w/metabolite-identification