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© 2021 MJH Life Sciences and Pharmaceutical Technology. All rights reserved.
© 2021 MJH Life Sciences™ and Pharmaceutical Technology. All rights reserved.
Jody Clark is vice president of sales and marketing at Selerity Technologies, 2484 West Custer Road, Salt Lake City, Utah 84104, USA. Tel. +1 801 978 2295 Fax +1 801 978 2298 firstname.lastname@example.org
May 01, 2004
Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the relationship between temperature and viscosity strongly influences the mobile phase flow profile and the diffusion characteristics. With temperature programming, thermal gradients mimic solvent gradients because of the changing polarity of the mobile phase and interactions with the stationary phase; often thermal gradients can replace solvent gradients.