Shimadzu’s Nexera

 Preparative Purification Liquid Chromatograph (LC). “Preparative HPLC” is a process in which specific substances are separated and purified from samples. The new Nexera Prep Series provides better prep processes for extraction of functional and impurity components, as well as purification of target compounds in the pharmaceutical, chemical and food industries. This product dramatically improves productivity via flexible scalability and an efficient, user friendly preparative workflow.

	Customer- and application-specific solution

 consists of multiple related products with the LC units as the core. Users select a pump, autosampler and detector to build a system according to the purpose of the preparative work. Systems can be configured using related products such as the LH-40 Liquid Handler, FRC-40 Fraction Collector, Shim-pack Scepter LC Column for Analysis and Preparative Work, and Column Hub.

	Preparative purification is generally performed following a multi-step workflow, which requires experience and time:

		Selection of system configuration and preparative column size based on the amount of purified sample or injection volume required

		Selection of fraction collector and container size according to the intended purpose

		Investigation of separation conditions in analytical scale

		Optimization of preparative LC conditions

		Confirmation of the purity of collected fractions

	In addition to these steps, in some cases salts need to be removed or solvent needs to be evaporated after purification, requiring additional time and effort.

	Time-saving by simplified, fast operation

 reduces the time spent on examining conditions using the software’s fractionation simulation function. It also enables simplified, fast operation in transition from analytical to preparative scale, with a column portfolio that covers a wide range of sizes. Furthermore, Shimadzu’s unique UFPLC (Ultra Fast Preparative and Purification LC) high-purity purification system reduces the time required for powderization via the elution of desalted target components with an organic solvent. This product satisfies a number of user demands, such as efficient desalting and powderization, multi-sample and multi-fraction analysis, effective separation at low cost and the ability to check the purity.

	Specifically, the product can be used for the following purposes:

		Concentration and purification of immunosuppressive agents

		Preparative work for impurities in degradants of medicinal agents

		Separation and purification of functional components from natural materials

		Consecutive analysis to separate and purify polymers and their additives and confirm the purity

 provides new features to simplify the preparative workflow:

	1.Reduces the time required for investigating conditions

	With the fractionation simulation function of the LabSolutions analysis software and the Shim-pack Scepter LC column for analytical and preparative HPLC, which offers excellent scalability, the product reduces the time required for investigating preparative work conditions. The LH-40 Liquid Handler can be used both, as a fraction collector and as an autosampler for large-volume injection with liquid surface detection function (option). It also enables seamless purity checks through re-injection (option) of collected sample after fractionation. Additional features including a sample rescue protocol to collect the sample from the system if an error occurs after an injection, a function to automatically extract the target peaks only, and high-purity preparative purification using detector signals from up to four channels, enable more efficient operation.

	2. UFPLC high-purity purification system

	Samples must be in a state of concentrated solution or dry powder to analyze their structure or to store them. When a solution contains water, it takes time to dry and concentrate it. When a salt such as trifluoroacetate has been added, it remains in the crystal of the compound, lowering the purity and negatively influencing pharmacokinetic studies and other tests. Shimadzu’s unique, automated UFPLC purification system introduces fractions containing the target compound, separated in a preparative column, into a trap column. Salt and water are then washed off and elution is induced with an organic solvent. This reduces the time required for drying, providing compounds with a high level of purity.

	Both the LH-40 Liquid Handler and FRC-40 Fraction Collector are designed to save installation space, requiring only half the space of other vendors’ products. They support containers of a form and size suitable for the recovered quantity. Up to six units can be connected, so up to 3240 10 mm diameter test tubes can be placed. Using the new Column Hub, up to six columns and four flow-line switching valves can be installed, enabling the compact configuration of various systems.

Articles

Shimadzu has introduced its new Nexera Prep Series Preparative Purification Liquid Chromatograph (LC). “Preparative HPLC” is a process in which specific substances are separated and purified from samples. 

New Nexera Prep Better Prep!

	Shimadzu’s new Nexera Ultra High-Performance Liquid Chromatograph series, incorporates artificial intelligence as Analytical Intelligence, allows systems to detect and resolve issues automatically. The 

 makes lab management simple by integrating IoT and device networking, enabling users to easily review instrument status, optimize resource allocation, and achieve higher throughput.

 maximizes reliability and uptime with fully unattended workflows that span from startup to shut-down. Operators can set the Nexera to start up at a specified time, so that it can complete auto-purge, equilibration, baseline checks and system suitability in advance, and be ready for analysis before they arrive at the lab. In addition, FlowPilot ramps up the flow rate gradually, reducing the possibility of damage to columns. The Nexera also has auto-diagnostics and auto-recovery capabilities that allow it to monitor pressure fluctuations to check for anomalies.

	With remote mobile phase monitoring and integrated consumables management, the system maximizes uptime and reliability. Real-time monitoring of mobile phase levels allows lab personnel to efficiently run batches and respond accordingly if there isn’t enough mobile phase before starting a run. In addition, Nexera tracks consumable usage and sends alerts when parts need replacing, allowing users to keep the system running at peak performance. The 

 allows analysts to confirm parameters and monitor chromatograms in real time directly from a web browser on their smart device.

	The Nexera UHPLC increases efficiency by automating workflows and maximizing throughput analysis. The SIL-40 autosampler can process the entire injection cycle time in as little as seven seconds, and continuous analysis can be carried out on up to 44 MTPs (using 3 plate changers). The SIL-40’s plate changers enable non-stop temperature-controlled analysis of thousands of samples. The Nexera automatically blends mobile phases at any set ratio, which speeds up the preparation of buffer solutions and the dilution of solvents. Because it prepares the exact amounts required for analysis, the Nexera reduces waste and labor.

	The Nexera’s elegant, compact design saves valuable bench space. Because it uses over 80 % less electricity when on standby, it significantly reduces running costs and supports an environmentally-friendly lab.

Shimadzu’s new Nexera Ultra High-Performance Liquid Chromatograph series, incorporates artificial intelligence as Analytical Intelligence, allows systems to detect and resolve issues automatically

New Nexera series LC-40Groundbreaking technology in terms of intelligence, efficiency and design

	Shimadzu’s Nexera Method Scouting system provides an outstanding platform for efficient HPLC method development and implementation. It is based on the 

  next-generation ultra-high performance liquid chromatograph, an all-round LC system for ultra-high-speed analysis.

 is equipped with two quaternary solvent pumps, a high pressure resistant column switching valve and the dedicated Method Scouting Solution control software to enable automated, quick and simple column and solvent screening. With its solvent blending capability and automated workflow, the system reduces preparation time and the risk of human error to a minimum when setting up the method and analysis schedule manually.

 systems can be used for a wide range of applications, from verifying the purity of synthetic compounds to quantifying their impurities, e.g. in pharmaceutical CMC and drug development/synthesis. In combination with the SPD-M30A photodiode array detector, Nexera X2 offers highly sensitive detection and a large dynamic range for quantification of high and low concentrations in a single experiment.

 Solution is the system’s dedicated control software which supports fast and secure operation of the Nexera Method Scouting System. It offers a graphical user interface to simplify the system set up as well as pre-configured system settings conforming to laboratory policies to provide reliable data while minimizing the risk of human error. Connecting between 

 for data evaluation and the DryLab®4 HPLC modeling software for strategic method optimization enables fast and efficient setup of methods as well as easy calculation and visualization of the design space. In combination with the VALIDAT© toolbox for method validation, the Nexera Method Scouting system provides an all-round solution for highly effective HPLC method development and implementation.

 4 is a registered trademark of Molnar Institute of Applied Chromatography
	Validat

Shimadzu’s Nexera Method Scouting system provides an outstanding platform for efficient HPLC method development and implementation. It is based on the Nexera X2 next-generation ultra-high performance liquid chromatograph, an all-round LC system for ultra-high-speed analysis.